Ana
Pego
Assistant Professor
Education
Aug 2014 – Aug 2018 UNIVERSITY OF SÃO PAULO
PhD in Toxicology
Thesis title: Investigation on the relationship between violent death, cocaine abuse and single nucleotide polymorphisms
Sep 2012 – Sep 2013 THE UNIVERSITY OF GLASGOW
Masters in Forensic Toxicology
Dissertation title: Evaluation of liquid phase microextraction techniques in forensic toxicology
Sep 2008 – Aug 2012 GLASGOW CALEDONIAN UNIVERSITY
Graduation in Forensic Investigation
Dissertation title: Evaluation of the antimicrobial effects of colloidal silver
Bio
Ana Pego obtained her undergraduate degree in Forensic Investigation, with a first-class honours, from Glasgow Caledonian University (in addition to an exchange period of 6 months at Tampere University of Applied Sciences), in 2012, where she has developed a final-year project on the properties of colloidal silver and their implementation for the production of antimicrobial polymers to be used in children's toys. After that, she decided to specialize in Forensic Toxicology and was awarded her masters degree from The University of Glasgow in 2013 where she graduated with merit and worked on evaluating different liquid-phase microextraction techniques (DISDME, LLLME and LPME) in forensic toxicology. She then obtained her Ph.D. from the University of Sao Paulo in 2018, after spending 3 months at the University of California, Los Angeles. During those 4 years, Dr. Pego investigated the relationship between violent death, cocaine abuse and SNPs where she has worked with postmortem cases and analysed both hair and blood using GC-MS and LC-MS/MS with a variety of extraction techniques. That same year, Dr. Pego joined Avans University of Applied Sciences, where she concluded her Post-Doc in 2020, after working on a project entitled: 'SherLOK: profiling of individuals through forensic analysis and imaging of hair'. During that time she has spent 6 months as a visiting scholar at Maastricht University developing a MALDI-TOF method for the analysis of single hair strands to test for capsaicins. She then worked for the Dutch Screening Group as Head of Development. Nowadays, Dr. Pego works as an Assistant Professor in the Sciences Department at John Jay college of Criminal Justice, City University of New York.
Her research focuses on innovative approaches to drug detection and biomonitoring, with a particular emphasis on hair analysis for the retrospective identification of drugs of abuse, novel psychoactive substances (NPS) and PFAS. Her work is also focused on developing strategies to differentiate external contamination from true drug incorporation. Dr. Pego’s work integrates advanced analytical techniques, including LC–MS/MS, with real-world applications in forensic and public health contexts. She currently collaborates with international organizations and agencies on drug monitoring initiatives.
JJC Affiliations
Courses Taught
Current courses
Undergraduate
TOX 416 Analytical Toxicology (Lecture & Laboratory)
TOX 425 Techniques of Analytical Toxicology
TOX 426 Analytical and Quantitative Toxicology Laboratory
Graduate
FOS 725 Forensic Toxicology I
Professional Memberships
1. The International Association of Forensic Toxicologists - TIAFT (Young Scientist Committee Member)
2. Society of Forensic Toxicologists – SOFT
3. American Academy of Forensic Sciences – AAFS
4. Portuguese Association of Forensic Sciences - APCF
5. Brazilian Society of Toxicology – SBTOX
6. Society of Hair Testing – SoHT
7. International society for the study of emerging drugs
8. European Society of Criminology – ESC
Languages
Scholarly Work
1. A.M.F. Pego, M. Schoffner1, V. R. Sammeta, M. Naeem, D. R. Manke, A. Chadeayne, G. C. Glatfelter, M. H. Baumann, M. Concheiro (2025) Development and Validation of an Analytical Method for the Determination of Select 4-Position Ring-Substituted Tryptamines in Plasma by Liquid Chromatography-Tandem Mass Spectrometry. Journal of Analytical Toxicology. 10.1093/jat/bkaf045
2. A. Capasso, A. L. Stotts, M. A. Xu, B. D. Manuel, S. Zelaya, S. J. Casillas, Y. R. Villarreal, A. Dorow, J. L. Brown, S. Xu, M. Concheiro, A.M.F. Pego, G. M. Wingood, S. A. P. Beegle, R. J. DiClemente. (2025) Safe Start, A Hybrid Intervention to Reduce Alcohol Exposed Pregnancies: Protocol for a Randomized Controlled Trial. BMC Public Health. 10.1186/s12889-025-26044-2
3. A. Dombroski; M. Faure Betancourt, A. Alvarado; P. St. Fleur; M. Concheiro; A.M.F. Pego (2024) The influence of cosmetic treatments in the uptake of in-vitro cocaine contamination in hair. Drug Testing and Analysis. 10.1002/dta.3741
4. A.M.F. Pego, E.J. Knaven, A.P.C. van de Plas, J.F. Brouwers, E. Cuypers, B. Flynders, R.M.A. Heeren, A.C. van Asten, B. M. de Rooij (2024) Untargeted metabolomics for lifestyle biomarker discovery in human hair. Forensic Science International. 10.1016/j.forsciint.2024.111938
5. R.R. Viana, A.M.F. Pego, T.F. Oliveira, E. Dallegrave, S. Eller. Liquid chromatography– tandem mass spectrometry method for simultaneous quantification of neurotransmitters in rat brain tissue exposed to 4′ -Fluoro-α-PHP (2022) Biomedical Chromatography. 10.1002/bmc.5487
6. P.R.Tomaz, T.S. Gonçalves, J.R. Santos, J. Scholz, T.O. Abe, P.A. Gaya, E. Figueiredo, H.H. Faria, I. Martins, A.M.F. Pego, B.A. Paranhos, M. Yonamine, A. Pereira, P.C.J.L. Santos (2022) Evaluation of the nicotine metabolite ratio in smoking patients treated with varenicline and bupropion. Frontiers in Pharmacology. 10.3389/fphar.2022.900112
7. L. DeFreitas, A.M.F. Pego, R. Kronstrand, E. Lendoiro, A. Castro-Ríos, M. Concheiro. Fast and Sensitive Method for the Determination of 17 Designer Benzodiazepines in Hair by Liquid Chromatography Tandem Mass Spectrometry (2022) Journal of Analytical Toxicology. 10.1093/jat/bkac044
Honors and Awards
Honors
1. Merit award by the University of Glasgow (2013)
2. First Class Honors award by Glasgow Caledonian University (2012)
Awards
1. Distinguished Teaching Prize (2025)
2. MacNamara Award (2024)
3. Santander Mobility scholarship award - International exchange (2017)
4. The International Association of Forensic Toxicologists (TIAFT) award - Conference Travel Winner (2016)
5. The International Union of Toxicology (IUTOX) and the Brazilian Society of Toxicology (SBTOX) award - Fellowship Travel Award Winner (2015)
6. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) award - Full financial support during PhD studies (2014 – 2018)
7. ERASMUS scholarship award - Studying and living abroad (2010)
8. Student Awards Agency for Scotland (SAAS) scholarship award - Fully paid undergraduate studies (2008 - 2012)
Research Summary
- Hair analysis of DoA and NPS
- Development of decontamination protocols
- Development and validation of forensic toxicology-related methodology
- Biomonitoring of drugs for public policies